Identification of Superoxide Dismutase (SOD) Isozymes in Plant Tissues.

Superoxide and hydrogen peroxide are reactive oxygen species (ROS) involved in the oxidation of multiple biological molecules and the signaling processes during plant growth and stress response. Thus, control of ROS is fundamental for cell survival and development, with superoxide dismutase (EC 1.15.1.1, SOD) being one of the main enzymes involved. Different isoforms of SOD catalyze the dismutation of superoxide (O2.-) to hydrogen peroxide (H2O2) and oxygen (O2), such as Mn-SODs, Cu,Zn-SODs, and Fe-SODs. Using non-denaturing polyacrylamide gel electrophoresis (PAGE) combined with a specific staining method for SOD activity, the protocol describes the identification of different SOD isozymes, based on their differential inhibition by KCN and H2O2, in different organs and plant species such as pea (Pisum sativum L.) leaves and pepper (Capsicum annuum L.) fruits.

Characterization in end-of-life care of older people attended by geriatricians / Caracterización de la atención al final de la vida de personas mayores atendidas por geriatría

Background and objective Symptom control at the end of life is essential, and palliative sedation is a viable intervention option for the care of terminally ill patients. This study aims to characterize the elderly population receiving end-of-life care plans and their management with palliative sedation in a geriatric unit at a high complexity hospital. Materials and methods A cross-sectional study was conducted, and a descriptive analysis was performed. Medical records of 163 patients admitted to a high complexity hospital in Bogota, Colombia between January 2016 and December 2019 were reviewed. Results From 163, 141 patients received an end-of-life care plan, and 22 were managed with palliative sedation. The mean age was 84 years, the most frequent cause of death was respiratory infections and 44% of patients had a history of cancer. Prior to admission, functional decline and the presence of moderate to severe dementia were frequently found. About one in ten persons required palliative sedation, which lasted an average of 2.22±5 days. The most common refractory symptom was dyspnea (45.45%), followed by pain (36.36%). Conclusions Palliative sedation is prevalent in the elderly population and characterizing this population can provide increased knowledge to improve end-of-life care (AU)

Antecedentes y objetivos El control de síntomas al final de la vida es fundamental, y la sedación paliativa resulta una opción de intervención en el cuidado de pacientes con enfermedades terminales. El objetivo es caracterizar una población de personas mayores que recibieron un plan de atención del final de la vida, incluyendo sedación paliativa en una unidad de geriatría de un hospital de alta complejidad. Materiales y métodos Estudio de corte transversal, se realizaron análisis descriptivos y se utilizaron métodos de acuerdo con el tipo de variable. Se revisaron las historias clínicas de 163 pacientes entre enero de 2016 y diciembre de 2019 de un hospital de alta complejidad en Bogotá, Colombia. Resultados Sobre 163 pacientes, 141 recibieron plan de atención de final de vida y 22 fueron manejados con sedación paliativa. La edad promedio fue de 84 años y el 58% eran mujeres. La causa de muerte más frecuente fue respiratoria infecciosa; el 44% tenían antecedente oncológico. La declinación funcional previa al ingreso y la presencia de demencia moderada o severa fueron condiciones que frecuentemente se encontraron en quienes se reorientó el esfuerzo terapéutico. Una de cada 10 personas requirió sedación paliativa, cuya duración fue de 2,22±5 días, el síntoma refractario más frecuente fue la disnea (45,45%), seguido de dolor (36,36%). Conclusiones La sedación paliativa resulta frecuente en la población mayor con enfermedades no oncológicas. La caracterización de estas personas promueve el aumento del conocimiento y la preparación para mejorar el manejo del final de la vida (AU)

Percepción de dirigentes deportivos locales y funcionarios municipales sobre políticas públicas deportivas locales de la comuna de Linares

La aplicación de las políticas públicas deportivas locales (PPDL) de la comuna de Linares se han visualizado gracias a la aplicación del Plan de Desarrollo Comunal de Actividad Física y Deportes (PDC AFyD) en el período 2018 - 2022 en la comunidad y las organizaciones deportivas locales (OODD), así como de funcionarios del propio municipio. Para conocer la percepción sobre la aplicación de PPDL se aplica una entrevista semiestructurada y confeccionada para tales efectos, a 28 dirigentes deportivos y 18 funcionarios municipales, de un universo muestral total de 127 posibles. Los individuos indican que, pese a ver cambios favorables en el período desconocen el proceso y, por ende, el mecanismo aplicado para el cambio, lo que despotencia la comprensión de la PPDL y el desarrollo de ésta por medio de las propias OODD y el municipio. La aplicación de las PPDL por medio de sus distintas herramientas e instrumentos requiere que se desarrolle bajo un proceso de consulta, pertinencia y complementación entre municipio, OODD, mundo educativo y vecinos beneficiarios; todo dentro de un marco de comunicación e información concreta y permanente que logre involucrar a todos los actores, brindando con ello una cuota de responsabilidad en su ejecución, control y evaluación.

The application of local public policies on sports (LPSP) of the commune of Linares has been visualized thanks to the application of the Communal Development Plan for Physical Activity and Sports (CDP PA&S) in the period 2018 - 2022 in the community and local sports organizations (LSO), as well as officials of the municipality itself. To know the perception of the implementation of LPSP, a semi-structured interview was applied to 28 sports leaders and 18 municipal officials out of a total sample universe of 127 possible. The individuals indicate that, despite seeing favorable changes in the period, they are unaware of the process and, therefore, the mechanism applied for change, which undermines the understanding of the LPSP and its development through the LSOO and the municipality. The application of the LPSP through its different tools and instruments requires that it be developed under a process of consultation, relevance, and complementarity between the municipality, LSOO, the educational world and beneficiary neighbors; all under a framework of communication and concrete and permanent information that manages to involve all the actors, thus providing a share of responsibility in its execution, control, and evaluation.

A implementação das políticas esportivas públicas locais (PEPL) na comuna de Linares foi visualizada graças à implementação do Plano de Desenvolvimento Comunitário para Atividade Física e Esporte (PDC AFyE) no período 2018 - 2022 na comunidade e organizações esportivas locais (OOEE), bem como funcionários do próprio município. Foi realizada uma entrevista semi-estruturada com 28 líderes esportivos e 18 funcionários municipais de um universo de amostra total de 127. Os indivíduos indicam que, apesar de verem mudanças favoráveis no período, não conhecem o processo e, portanto, o mecanismo aplicado para a mudança, o que prejudica a compreensão do PEPL e seu desenvolvimento através do OOEE e do próprio município. A implementação do PEPL através de suas diferentes ferramentas e instrumentos requer um processo de consulta, relevância e complementaridade entre o município, o OOEE, o mundo educacional e os vizinhos beneficiários; tudo isso dentro de uma estrutura de comunicação e informação concreta e permanente que envolva todos os atores, proporcionando assim uma parcela de responsabilidade em sua implementação, controle e avaliação.

Diversity of rickettsiae in ticks (Acari: Ixodidae) collected from wild vertebrates in part of the Amazon, Cerrado, and Pantanal biomes in Brazil.

Ticks parasitizing 102 wild animals in the states of Mato Grosso and Goiás, Brazil were collected between 2015 and 2018. A total of 2338 ticks (865 males, 541 females, 823 nymphs, and 109 larvae) belonging to four genera (Amblyomma, Dermacentor, Haemaphysalis, and Rhipicephalus) and at least 21 species were identified. DNA extraction and a molecular survey for rickettsial agents were performed on 650 ticks. The results revealed parasitism by the following species: Rickettsia amblyommatis in Amblyomma cajennense s.s., A. cajennense s.l., Amblyomma coelebs, Amblyomma humerale, Amblyomma longirostre, Amblyomma nodosum, Amblyomma scalpturatum, Amblyomma sculptum, and Amblyomma romitii; Rickettsia parkeri in Amblyomma nodosum, Amblyomma ovale, Amblyomma scalpturatum, and Amblyomma triste; Rickettsia rhipicephali in Haemaphysalis juxtakochi; Rickettsia sp. in A. cajennense s.s., A. nodosum, and A. sculptum, and lastly, 'Candidatus Rickettsia andeanae' in Amblyomma parvum and Rhipicephalus microplus. This study expands the body of knowledge about tick parasitism among wild animals, including new data concerning tick-host associations, and provides information about the epidemiology of tick-borne pathogens in the Center-West region of Brazil.

Characterization in end-of-life care of older people attended by geriatricians.

BACKGROUND AND OBJECTIVE: Symptom control at the end of life is essential, and palliative sedation is a viable intervention option for the care of terminally ill patients. This study aims to characterize the elderly population receiving end-of-life care plans and their management with palliative sedation in a geriatric unit at a high complexity hospital. MATERIALS AND METHODS: A cross-sectional study was conducted, and a descriptive analysis was performed. Medical records of 163 patients admitted to a high complexity hospital in Bogota, Colombia between January 2016 and December 2019 were reviewed. RESULTS: From 163, 141 patients received an end-of-life care plan, and 22 were managed with palliative sedation. The mean age was 84 years, the most frequent cause of death was respiratory infections and 44% of patients had a history of cancer. Prior to admission, functional decline and the presence of moderate to severe dementia were frequently found. About one in ten persons required palliative sedation, which lasted an average of 2.22±5 days. The most common refractory symptom was dyspnea (45.45%), followed by pain (36.36%). CONCLUSIONS: Palliative sedation is prevalent in the elderly population and characterizing this population can provide increased knowledge to improve end-of-life care.

The effects of temperature and salinity on the blood chemistry and survival of juvenile Atlantic tarpon Megalops atlanticus.

Atlantic tarpon Megalops atlanticus are highly migratory sportfish that support recreational fisheries throughout their range. In US waters, juveniles can be found in coastal and estuarine habitats along the Gulf of Mexico and Atlantic seaboard, with temperature limiting their northern latitudinal distribution. Juveniles may overwinter in these areas during the first several years of life. Low temperatures are known to cause mortality in adults, but the challenges of temperature are less understood for juveniles. Furthermore, salinity, which can change dramatically in these habitats, may have a synergistic effect with temperature. To examine the physiological effects of temperature and salinity on juvenile tarpon, wild fish were acclimated to a range of conditions that potentially occur in the northern range of their estuarine habitats. The haematology of juvenile tarpon was examined in two salinity (≤2 and ≥30 ppt) and temperature (15 and 25°C) treatments, followed by a low-temperature tolerance test. After 2 weeks in treatment conditions, blood samples were analysed for haematocrit, pH, red blood cell concentration, haemoglobin content and plasma osmolality. Increased plasma osmolality was observed in fish at low temperature (15°C compared to 25°C) and at high salinity (≥30 ppt compared to ≤2 ppt). Blood pH was increased at 15°C compared to 25°C, with the highest pH at 15°C and low salinity. Haemoglobin, haematocrit and red blood cell concentration were higher at 25°C than 15°C, with haemoglobin lowest at 15°C and low salinity. For the low-temperature tolerance test, all fish were acclimated to 15°C for 2 weeks, then transferred to separate tanks where temperature was gradually decreased at 0.9 ± 0.1°C/h until fish lost equilibrium. Fish at low salinity lost equilibrium more rapidly (1 ppt, 12.65 ± 0.46°C) than fish at high salinity (30 ppt, 11.26 ± 0.14°C). The results indicate juvenile tarpon are susceptible to low temperature, which is exacerbated by low salinity, findings useful in the assessment of juvenile tarpon overwintering habitat.

Health promotion practices in primary care: comparison between florianópolis-brazil and girona-spain / Prácticas de promoción de la salud en atención primaria: comparación entre florianópolis-brasil y girona-espanha / Práticas de promoção da saúde na atenção primária: comparativo entre florianópolis-brasil e girona-espanha

ABSTRACT Objective: to understand the health promotion practices developed by nurses in the Primary Care context in Florianópolis and Girona. Method: this is a comparative study with a qualitative approach and of the descriptive exploratory type, carried out with eight nurses between June 2021 and April 2022 in health units that developed health promotion practices. The data, collected by means of semi-structured interviews, were analyzed through thematic analysis based on the health promotion framework. Results: four categories related to health promotion practices emerged from the data, namely: Training actions for health professionals in health promotion; Health promotion activities in individual consultations; Health education group activities; and Community health promotion actions. Conclusion: it is concluded that, in both municipalities, nurses develop individual and collective health promotion practices through groups and community actions, focusing on lifestyle changes. In Florianópolis they are grounded on the National Health Promotion Policy and, in Girona, health promotion actions are based on actions involving specific groups aimed at preventing diseases.

RESUMEN Objetivo: comprender las prácticas de promoción de la salud desarrolladas por enfermeros en el contexto de Atención Primaria de Florianópolis y Girona. Método: estudio comparativo de enfoque cualitativo y del tipo exploratorio-descriptivo, realizado entre junio de 2021 y abril de 2022 con ocho enfermeros en unidades de salud que desarrollaban prácticas de promoción de la salud. Los datos, recolectados por medio de entrevistas semiestructuradas, fueron analizados a través de análisis temático sobre la base del referencial de la promoción de la salud. Resultados: cuatro categorías relacionadas con las prácticas de promoción de la salud surgieron de los datos, a saber: Acciones para la formación de profesionales de la salud en promoción de la salud; Actividades de promoción de la salud en consultas individuales; Actividades grupales de educación en salud; y Acciones comunitarias de promoción de la salud. Conclusión: se concluye que, en ambos municipios, los enfermeros desarrollan prácticas individuales y colectivas de promoción de la salud por medio de grupos y acciones comunitarias, con énfasis en modificar estilos de vida. En Florianópolis se fundamentan en la Política Nacional de Promoción de la Salud y, en Girona, las acciones de promoción de la salud se basan en actividades relacionadas con grupos específicos dirigidos a prevenir enfermedades.

RESUMO Objetivo: compreender as práticas de promoção da saúde desenvolvidas por enfermeiros no contexto da Atenção Primária de Florianópolis e Girona. Método: trata-se de um estudo comparativo, de abordagem qualitativa, do tipo exploratório descritivo, realizado em unidades de saúde que desenvolviam práticas de promoção da saúde, com oito enfermeiros, no período entre junho de 2021 a abril de 2022. Os dados, coletados por entrevistas semiestruturadas, foram analisados por meio de análise temática com base no referencial da promoção da saúde. Resultados: emergiram dos dados quatro categorias relacionadas às práticas de promoção da saúde: Ações de formação de profissionais de saúde em promoção da saúde; Atividades de promoção da saúde na consulta individual; Atividades grupais de educação em saúde; Ações comunitárias de promoção da saúde. Conclusão: conclui-se que em ambos os municípios os enfermeiros desenvolvem práticas de promoção da saúde individuais e coletivas por meio de grupos e ações comunitárias, com enfoque na mudança de estilos de vida. Em Florianópolis estão fundamentadas na Política Nacional de Promoção da Saúde e, em Girona, as ações de promoção da saúde estão baseadas nas ações envolvendo grupos específicos voltados para a prevenção da doença.

Potentialities and challenges for care in the primary health care context / Potencialidades y desafíos para la asistencia en el contexto de la atención primaria de la salud / Potencialidades e desafios para a assistência no contexto da atenção primária à saúde

ABSTRACT Objective: to understand the potentialities and challenges for the care provided by professionals in the primary health care context. Method: a qualitative research study of the participatory action type, based on Paulo Freire's Research Itinerary, which consists of Thematic Investigation; Coding and Decoding; and Critical Unveiling. It was developed in the first half of 2021, with the participation of 20 professionals, including physicians, nurses, dentists, nursing technicians, dental assistants and community agents, all from a Basic Health Unit in Santa Catarina. Results: the health professionals revealed the team's engagement as potentialities, as well as welcoming, which strengthens interpersonal relationships in Primary Care. As challenges, they highlighted the limited professional appreciation and scarce material and human resources, factors that generate tensions for the assistance to be provided. Conclusion: engagement and interpersonal relationships reveal the professionals' commitment and dedication to promoting comprehensive and good quality care, seeking to overcome the limitations inherent to assistance in Primary Health Care.

RESUMEN Objetivo: comprender las potencialidades y los desafíos para la asistencia desarrollada por los profesionales en el contexto de la Atención Primaria de la Salud. Método: investigación cualitativa y del tipo acción participante, fundamentada en el Itinerario de Investigación de Paulo Freire, que consiste en Investigación Temática; Codificación y Decodificación; y Revelación Crítica. Se desarrolló en el primer semestre de 2021 con la participación de 20 profesionales, entre médicos, enfermeros, odontólogos, técnicos de Enfermería, auxiliares de consultorio dental y agentes comunitarios, todos de una Unidad Básica de Salud de Santa Catarina. Resultados: los profesionales de la salud indicaron lo siguiente como potencialidades: compromiso del equipo, al igual que la recepción, que fortalece las relaciones interpersonales en la Atención Primaria. Como desafíos, destacaron la limitada valorización profesional y escasos recursos materiales y humanos, factores que generan tensiones sobre la asistencia. Conclusión: el compromiso y las relaciones interpersonales revelan la dedicación de los profesionales por promover una atención integral y de buena calidad, procurando suplir las limitaciones de la asistencia provista en la Atención Primaria de la Salud.

RESUMO Objetivo: compreender as potencialidades e desafios para a assistência desenvolvida pelos profissionais no contexto da atenção primária à saúde. Método: pesquisa qualitativa, do tipo ação participante, fundamentada no Itinerário de Pesquisa de Paulo Freire, que consiste de Investigação Temática; Codificação e Descodificação; e, Desvelamento Crítico. Foi desenvolvida no primeiro semestre de 2021, com a participação de 20 profissionais, entre médico, enfermeiro, odontólogo, técnico de enfermagem, auxiliar de consultório dentário e agente comunitário, todos de uma Unidade Básica de Saúde de Santa Catarina. Resultados: os profissionais de saúde desvelaram como potencialidades o engajamento da equipe, assim como o acolhimento que fortalece as relações interpessoais na atenção primária. Como desafios ressaltaram a limitada valorização profissional e parcos recursos materiais e humanos, fatores esses que geram tensionamentos para a assistência. Conclusão: o engajamento e a relação interpessoal revelam o comprometimento e dedicação dos profissionais em promover uma atenção integral e de qualidade, buscando suprir as limitações da assistência na atenção primária à saúde.

Retrospective molecular investigation of Mayaro and Oropouche viruses at the human-animal interface in West-central Brazil, 2016-2018.

Mayaro virus (MAYV, Togaviridae) and Oropouche orthobunyavirus (OROV, Peribunyaviridae) are emerging enzootic arboviruses in Latin America. Outbreaks of febrile illness associated with MAYV and OROV have been reported among humans mainly in the northern region of Brazil since the 1980s, and recent data suggest these viruses have circulated also in more populated areas of western Brazil. MAYV shares mosquito vectors with yellow fever virus and it has been historically detected during yellow fever epidemics. Aiming to investigate the transmission of OROV and MAYV at the human-animal interface during a yellow fever, chikungunya and Zika outbreaks in Brazil, we conducted a retrospective molecular investigation in 810 wild and domestic animals, 106 febrile patients, and 22.931 vectors collected from 2016 to 2018 in Cuiaba and Campo Grande metropolitan regions, western Brazil. All samples tested negative for OROV and MAYV RNA by RT-qPCR. Findings presented here suggest no active circulation of MAYV and OROV in the sampled hosts. Active surveillance and retrospective investigations are instrumental approaches for the detection of cryptic and subclinical activity of enzootic arboviruses and together serve as a warning system to implement appropriate actions to prevent outbreaks.

Serviço infantojuvenil para usuários de drogas: clientela e atendimento / Child and youth service for drug users: clientele and service

O estudo intenta conhecer o perfil de crianças e adolescentes atendidos em serviço comunitário para usuários de drogas e analisar as características do atendimento oferecido. Estudo quantitativo, descritivo-exploratório, com análise descritiva dos dados de 115 prontuários de um CAPSi especializado em drogas. Foi utilizada frequência válida, devido aos dados não informados. A maioria era de homens (78,2%) com idade média de 14,7 anos (dp = 1,98). Mais da metade não frequentava a escola (54,2%) e 33,7% residia com a mãe. Em 81,9% dos prontuários algum familiar fazia uso abusivo de drogas, sendo 52,9% o pai. A maconha apresentou maior prevalência e início mais precoce. O serviço dispunha de atividades que buscavam abranger diferentes necessidades, entre elas, questões escolares e familiares da clientela, justificando a proposta de um serviço comunitário especializado no cuidado a crianças e adolescentes usuários de drogas. Entretanto, observou-se como maior desafio a continuidade do atendimento no serviço.

Biotic Indicators for Ecological State Change in Amazonian Floodplains.

Riverine floodplains are biologically diverse and productive ecosystems. Although tropical floodplains remain relatively conserved and ecologically functional compared to those at higher latitudes, they face accelerated hydropower development, climate change, and deforestation. Alterations to the flood pulse could act synergistically with other drivers of change to promote profound ecological state change at a large spatial scale. State change occurs when an ecosystem reaches a critical threshold or tipping point, which leads to an alternative qualitative state for the ecosystem. Visualizing an alternative state for Amazonian floodplains is not straightforward. Yet, it is critical to recognize that changes to the flood pulse could push tropical floodplain ecosystems over a tipping point with cascading adverse effects on biodiversity and ecosystem services. We characterize the Amazonian flood pulse regime, summarize evidence of flood pulse change, assess potential ecological repercussions, and provide a monitoring framework for tracking flood pulse change and detecting biotic responses.

Considering the influence of land use/land cover on estuarine biotic richness with Bayesian hierarchical models.

The composition of land use/land cover (LULC) in coastal watersheds has many implications for estuarine system ecological function. Land use/land cover can influence allochthonous inputs and can enhance or degrade the physical characteristics of estuaries, which in turn affects estuaries' ability to support local biota. However, these implications for estuaries are often poorly considered when assessing the value of lands for conservation. The focus of research regarding terrestrial and estuarine interfaces often evaluates how LULC may stress estuarine ecosystems, but in this study we sought to understand how LULC may both positively and negatively affect estuaries using measures of observed biotic richness as proxies for estuarine function. We investigated the influence of LULC on estuarine biotic richness with Bayesian hierarchical models using multiple geospatial data sets from 33 estuaries and their associated watersheds along the Gulf of Mexico coastal region of the United States. We designed the hierarchical models with observed species richness of three functional groups (FGs) (i.e., pelagic fishes, forage fishes, and shrimp) from fishery-independent trawl surveys as response variables. We then set salinity and water temperature as trawl-specific covariates and measures of influence from six LULC classes as estuary-specific covariates and allowed the models to vary by estuary, trawl program, salinity, and temperature. The model results indicated that the observed richness of each FG was both positively and negatively associated with different LULC classes, with estuarine wetlands and forested lands demonstrating the strongest positive influences on each FG. The results are generally consistent with past studies, and the modeling framework provides a promising way to systematically quantify LULC linkages with the biotic health of estuaries for the purposes of potentially valuing the estuarine implications of land conservation.

Characterization of personal injuries in the stomatognathic system assessed at the Instituto Nacional de Medina Legal y Ciencias Forenses- Regional Suroccidente between 2015 and 2020 / Caracterización de las lesiones personales en el sistema estomatognático valoradas en el Instituto Nacional de Medina Legal y Ciencias Forenses, Regional Suroccidente, entre 2015 y 2020

Introduction: Personal injury forensic reports diagnose and define the damage to the body and health of a person including the stomatognathic system by injuries produced as the consequence of other people's actions. Objective: To characterize the personal injuries affecting the stomatognathic system as a result of violent actions in 266 cases evaluated at the Instituto Nacional de Medina legal y Ciencias Forenses-Regional Suroccidente (Cali, Colombia) between 2015 and 2020. Materials and methods: We conducted a descriptive cross-sectional study to characterize the personal injuries affecting the stomatognathic system. The information was taken from the personal injuries forensic reports registered on the Colombian forensic registration system (Sistema de Información Forense de Clínica de Colombia, SICLICO) platform and associated with the nature of the injury including its consequences and the medico-legal incapacity issued. Results: We observed a higher frequency of male aggressors (94.4%) and male victims (64.3%), especially in the age group between 21 and 30 years (32.6%). The most frequent contexts were interpersonal violence (76.3%) and intimate partner violence (19.9%). The main aggression mechanism was blunt trauma (88.0%). The lesions reported in soft tissues (83.5%) affected periodontal tissues (48.9%) and lips (28.6%) due to edema (32.7%) and wounds (22.9%) while in hard tissues (55.1%), they affected the teeth (41.4%) due to complicated fractures in the cervical third (19.2%) and avulsion (18%). Definitive disability predominated (64.7%) followed by 20-day disability (28.6%) and functional sequelae (24.1%). Conclusions: Periodontal tissues and teeth were the most affected structures causing considerable disabilities, as well as functional and aesthetic sequelae that mainly affected men in productive age.

Introducción. El dictamen de lesiones personales hace precisiones sobre el daño ocurrido en el cuerpo o en la salud de una persona. La valoración del odontólogo es de gran importancia en los casos en que se ha visto afectado el sistema estomatognático. Objetivo. Caracterizar las lesiones personales que afectaron el sistema estomatognático como producto de actos violentos en 266 casos valorados en el Instituto Nacional de Medina Legal y Ciencias Forenses, Regional Suroccidente, entre 2015 y 2020. Materiales y métodos. Se hizo un estudio descriptivo de corte transversal de las lesiones personales que afectaron el sistema estomatognático. Se incluyó información del dictamen de lesiones personales obtenida de la plataforma del Sistema de Información Forense de Clínica de Colombia (SICLICO) y asociada con la naturaleza de la lesión, las secuelas y la incapacidad médico-legal. Resultados. Se observó una mayor frecuencia de agresores y de víctimas masculinas (94,4 y 64,3 %, respectivamente), especialmente en el grupo etario entre los 21 y los 30 años (32,6 %). Los contextos más frecuentes fueron los de violencia interpersonal (76,3 %) y violencia de pareja (19,9 %). El principal mecanismo de agresión fue el contundente (88,0 %). Las lesiones reportadas en tejidos blandos (83,5 %) afectaron tejidos periodontales (48,9 %) y labios (28,6 %) con edema (32,7 %) y heridas (22,9 %), en tanto que, en tejidos duros (55,1 %), afectaron los dientes (41,4 %) por fractura complicada en tercio cervical (19,2 %) y avulsión (18 %). Predominó la incapacidad definitiva (64,7 %), seguida de la de 20 días (28,6 %) y las secuelas funcionales (24,1 %). Conclusiones: Los tejidos periodontales y los dientes fueron las estructuras más afectadas, lo que ocasionó incapacidades considerables, y dejó secuelas funcionales y estéticas principalmente entre los hombres en edad productiva.

Proactively averting the collapse of Amazon fisheries based on three migratory flagship species.

Migratory species are the most important commercial fishes in the Amazon. They are also now the most threatened directly by some combination of overfishing, floodplain deforestation, and dam construction. Limited governmental monitoring and implemented regulations impede adequate management of the fisheries at adequate scale. We summarize the current stock status of the three most heavily exploited long-distance migratory species, which are two goliath catfishes (Brachyplatystoma rousseauxii and B. vaillantii) and the characiform Colossoma macropomum. In addition, we analyze impacts beyond overfishing on these species. Our results indicate: (i) the overfishing trends for these important species are either ominous or indicate the verge of collapse of the commercial fisheries based on them, and (ii) a dangerous synergy between overfishing, hydroelectric dams, and floodplain deforestation further challenge fisheries management of migratory species in the Amazon. We propose eight direct governmental actions as a proactive approach that addresses the main impacts on the fisheries. We consider that the most practical way to assess and manage overfishing of migratory species in the short run in an area as large as the main commercial fishing area in the Amazon is at market sites where enforced regulations can control fish catch. The management of the three species considered here has implications beyond just their sustainability. Their management would represent a paradigm shift where the governments assume their legal responsibilities in fishery management. These responsibilities include regulation enforcement, data collecting, inter-jurisdictional cooperation to protect migratory species at realistic life history scales, mitigation of the Madeira dams to assure goliath catfish passage to the largest western headwater region, and recognition of monitoring and managing wetland deforestation for the protection of fish and other aquatic and terrestrial biodiversity.

Caracterización de las lesiones personales en el sistema estomatognático valoradas en el Instituto Nacional de Medina Legal y Ciencias Forenses, Regional Suroccidente, entre 2015 y 2020 / Characterization of personal injuries in the stomatognathic system assessed at the Instituto Nacional de Medina Legal y Ciencias Forenses- Regional Suroccidente between 2015 and 2020

Introducción. El dictamen de lesiones personales hace precisiones sobre el daño ocurrido en el cuerpo o en la salud de una persona. La valoración del odontólogo es de gran importancia en los casos en que se ha visto afectado el sistema estomatognático. Objetivo. Caracterizar las lesiones personales que afectaron el sistema estomatognático como producto de actos violentos en 266 casos valorados en el Instituto Nacional de Medina Legal y Ciencias Forenses, Regional Suroccidente, entre 2015 y 2020. Materiales y métodos. Se hizo un estudio descriptivo de corte transversal de las lesiones personales que afectaron el sistema estomatognático. Se incluyó información del dictamen de lesiones personales obtenida de la plataforma del Sistema de Información Forense de Clínica de Colombia (SICLICO) y asociada con la naturaleza de la lesión, las secuelas y la incapacidad médico-legal. Resultados. Se observó una mayor frecuencia de agresores y de víctimas masculinas (94,4 y 64,3 %, respectivamente), especialmente en el grupo etario entre los 21 y los 30 años (32,6 %). Los contextos más frecuentes fueron los de violencia interpersonal (76,3 %) y violencia de pareja (19,9 %). El principal mecanismo de agresión fue el contundente (88,0 %). Las lesiones reportadas en tejidos blandos (83,5 %) afectaron tejidos periodontales (48,9 %) y labios (28,6 %) con edema (32,7 %) y heridas (22,9 %), en tanto que, en tejidos duros (55,1 %), afectaron los dientes (41,4 %) por fractura complicada en tercio cervical (19,2 %) y avulsión (18 %). Predominó la incapacidad definitiva (64,7 %), seguida de la de 20 días (28,6 %) y las secuelas funcionales (24,1 %). Conclusiones. Los tejidos periodontales y los dientes fueron las estructuras más afectadas, lo que ocasionó incapacidades considerables, y dejó secuelas funcionales y estéticas principalmente entre los hombres en edad productiva.

Introduction: Personal injury forensic reports diagnose and define the damage to the body and health of a person including the stomatognathic system by injuries produced as the consequence of other people's actions. Objective: To characterize the personal injuries affecting the stomatognathic system as a result of violent actions in 266 cases evaluated at the Instituto Nacional de Medina legal y Ciencias Forenses-Regional Suroccidente (Cali, Colombia) between 2015 and 2020. Materials and methods: We conducted a descriptive cross-sectional study to characterize the personal injuries affecting the stomatognathic system. The information was taken from the personal injuries forensic reports registered on the Colombian forensic registration system (Sistema de Información Forense de Clínica de Colombia, SICLICO) platform and associated with the nature of the injury including its consequences and the medico-legal incapacity issued. Results: We observed a higher frequency of male aggressors (94.4%) and male victims (64.3%), especially in the age group between 21 and 30 years (32.6%). The most frequent contexts were interpersonal violence (76.3%) and intimate partner violence (19.9%). The main aggression mechanism was blunt trauma (88.0%). The lesions reported in soft tissues (83.5%) affected periodontal tissues (48.9%) and lips (28.6%) due to edema (32.7%) and wounds (22.9%) while in hard tissues (55.1%), they affected the teeth (41.4%) due to complicated fractures in the cervical third (19.2%) and avulsion (18%). Definitive disability predominated (64.7%) followed by 20-day disability (28.6%) and functional sequelae (24.1%). Conclusions: Periodontal tissues and teeth were the most affected structures causing considerable disabilities, as well as functional and aesthetic sequelae that mainly affected men in productive age.

A manipulation of carotenoid metabolism influence biomass partitioning and fitness in tomato.

Improving yield, nutritional value and tolerance to abiotic stress are major targets of current breeding and biotechnological approaches that aim at increasing crop production and ensuring food security. Metabolic engineering of carotenoids, the precursor of vitamin-A and plant hormones that regulate plant growth and response to adverse growth conditions, has been mainly focusing on provitamin A biofortification or the production of high-value carotenoids. Here, we show that the introduction of a single gene of the carotenoid biosynthetic pathway in different tomato cultivars induced profound metabolic alterations in carotenoid, apocarotenoid and phytohormones pathways. Alterations in isoprenoid- (abscisic acid, gibberellins, cytokinins) and non-isoprenoid (auxin and jasmonic acid) derived hormones together with enhanced xanthophyll content influenced biomass partitioning and abiotic stress tolerance (high light, salt, and drought), and it caused an up to 77% fruit yield increase and enhanced fruit's provitamin A content. In addition, metabolic and hormonal changes led to accumulation of key primary metabolites (e.g. osmoprotectants and antiaging agents) contributing with enhanced abiotic stress tolerance and fruit shelf life. Our findings pave the way for developing a new generation of crops that combine high productivity and increased nutritional value with the capability to cope with climate change-related environmental challenges.

Revisión narrativa: unidad de ortogeriatría, la propuesta para reducir los desenlaces adversos en adultos mayores con fracturas de cadera por fragilidad / Narrative review: Orthogeriatrics unit, the proposal to reduce adverse outcomes in older adults with fragility fractures

Introducción Las fracturas de cadera son un problema de salud pública debido a su asociación con altas tasas de mortalidad a un año, deterioro funcional y empeoramiento de la calidad de vida. Un enfoque multidisciplinario mejora los resultados de los pacientes adultos mayores con fracturas por fragilidad. Objetivo Realizar una revisión narrativa para evaluar la evidencia de las unidades de ortogeriatría como estrategia para reducir desenlaces adversos en adultos mayores con fracturas de cadera por fragilidad. Materiales y métodos Se realizó una búsqueda de la literatura en las siguientes bases de datos: PubMed,Cochrane y Scielo, con los términos: "hip fracture" or "fragility fracture" AND "multidisciplinary team" or "Geriatric co management" or AND "orthogeriatric" or "ortho-geriatric". Resultados El manejo por ortogeriatría reduce el tiempo de evaluación pre-quirúrgico, complicaciones postoperatorias y la estancia hospitalaria. La valoración interdisciplinaria ha demostrado ser eficaz para disminuir el deterioro funcional, la mortalidad intrahospitalaria, la mortalidad a los 30 días y el riesgo de institucionalización en comparación con otros modelos de atención. Estos factores impactan sobre la optimización de recursos disminuyendo los costos en salud. Conclusión Los servicios de ortogeriatría son el modelo ideal para tratar las fracturas de cadera por fragilidad en adultos mayores. En Colombia se deben implementar más unidades de ortogeriatría para mejorar la atención intrahospitalaria, crear programas de seguimiento y rehabilitación.

Introduction Hip fracture is a public health problem due to its association with high mortality rates at one year, functional decline and worsening of quality of life. A multidisciplinary approach improves outcomes for elderly patients with fragility fracture. Objective To carry out a narrative review to evaluate the evidence of orthogeriatric units as a strategy to reduce adverse outcomes in older adults with fragility hip fractures. Material and Methods A literature search was carried out in the following databases: PubMed, Cochrane and Scielo, with the terms: "hip fracture" or "fragility fracture" AND "multidisciplinary team" or "Geriatric co management" or AND "orthogeriatric" or "ortho-geriatric". Results Care by orthogeriatrics reduces pre-operative surgical time, postoperative complications and hospital stay. Interdisciplinary assessment has been shown to be effective in reducing functional decline, in-hospital mortality, 30-day mortality, and the risk of institutionalization compared to other models of care. These factors impact on the optimization of resources, reducing health costs. Conclusion Orthogeriatric services are the ideal model to treat fragility fractures in older adults. In Colombia, more orthogeriatric units should be implemented to improve in-hospital care, create follow-up and rehabilitation programs.

Implicación clínica del protostílido: una mirada desde la antropología dental y la odontología no invasiva / Clinical implication of protostlylid: a point of view from dental anthropology and non-invasive dentistry

RESUMEN De acuerdo al modelo patológico multifactorial, la morfología dental ha sido asociada como uno de los factores etiológicos de la caries al favorecer el acumulo de restos alimenticios y la retención de biopelícula. Uno de los rasgos morfológicos dentales más frecuentes de la población colombiana es el punto P del protostílido, el cual se constituye en una fosa que se expresa en el surco de desarrollo vestibulomesial de los molares inferiores, región que le sigue a la superficie oclusal como uno de los sitios en donde se desarrollan con más frecuencia lesiones cariosas. Sin embargo, el desconocimiento de este rasgo morfológico por la mayoría de los odontólogos hace que el sistema morfológico del protostílido sea mal diagnosticado, lo que conlleva en muchos casos al sobretratamiento de dicha fosa con terapéuticas invasivas, las cuales, podrían ser evitadas con un conocimiento adecuado de la morfología dental y con un manejo preventivo o con técnicas no invasivas. Por tanto, el objetivo de esta revisión de tema es reconocer la expresión del punto P del protostílido y realizar una aproximación a las implicaciones clínicas del mismo y las posibilidades diagnósticas y terapéuticas conservadores que ofrece la odontología para controlar el acumulo de restos alimenticios y la retención de biopelícula.

ABSTRACT According to the multifactorial pathological model, dental morphology has been associated as one of the etiological factors of caries by favoring the accumulation of food remains and biofilm retention. One of the most frequent of non-metric dental traits of the Colombian population is the P point of the protostylid, which is constituted in a fossa that is expressed in the bucomesial development groove of the lower molars, a region that follows the occlusal surface as one of sites where carious lesions develop more frequently. However, the lack of knowledge of this morphological feature by most dentists makes the morphological system of the protostylid misdiagnosed, which in many cases leads to the overtreatment of this pit with invasive therapies, which could be avoided with a knowledge adequate dental morphology and with a preventive management or non-invasive techniques. Therefore, the aim of this review of the subject is to reconcile the expression of the P point of the protostylid and to make an approximation to the clinical implications of the same and the conservative diagnostic and therapeutic possibilities offered by dentistry to control the accumulation of food rests and retention of dental biofilm.

En homenaje al doctor Carlos Alfonso Mejía Pavony: Memoria y legado

"El maestro que camina, a la prestigiosa sombra de un templo, en medio de sus discípulos, no les da de su sabiduría, sino, que les transmite de su fe y de su amor, su disfrutar en la búsqueda. Pues si él, es verdaderamente sabio, él sabe que, no podrá hacerlos entrar en la casa de su sabiduría; él los conducirá, no obstante, hasta el umbral de tu propio espíritu". El Profeta (Gibran Jalil Gibran, 1923).

Reducing self-shading effects in Botryococcus braunii cultures: effect of Mg2+ deficiency on optical and biochemical properties, photosynthesis and lipidomic profile.

Microalgae biomass exploitation as a carbon-neutral energy source is currently limited by several factors, productivity being one of the most relevant. Due to the high absorption properties of light-harvesting antenna, photosynthetic cells tend to capture an excessive amount of energy that cannot be entirely channeled through the electron transfer chain that ends up dissipated as heat and fluorescence, reducing the overall light use efficiency. Aiming to minimize this hurdle, in this work we studied the effect of decreasing concentrations of Magnesium (Mg2+) on the chlorophyll a content, photosynthetic performance, biomass and lipid production of autotrophic cultures of Botryococcus braunii LB 572. We also performed, for the first time, a comparative lipidomic analysis to identify the influence of limited Mg2+ supply on the lipid profile of this algae. The results indicated that a level of 0.0037 g L-1 MgSO4 caused a significant decline on chlorophyll a content with a concomitant 2.3-fold reduction in the biomass absorption coefficient. In addition, the Mg2+ limitation caused a decrease in the total carbohydrate content and triggered lipid accumulation, achieving levels of up to 53% DCW, whereas the biomass productivity remained similar for all tested conditions. The lipidome analysis revealed that the lowest Mg2+ concentrations also caused a differential lipid profile distribution, with an enrichment of neutral lipids and an increase of structural lipids. In that sense, we showed that Mg2+ limitation represents an alternative optimization approach that not only enhances accumulation of neutral lipids in B. braunii cells but also may potentially lead to a better areal biomass productivity due to the reduction in the cellular light absorption properties of the cells.